Blogs

10
Jan 2026

Managing False Positives and False Negatives in Deterministic CCIT Systems

Managing-False-Positives-and-False-Negatives-in-Deterministic-CCIT-Systems

Container Closure Integrity Testing (CCIT) is a critical quality assurance activity for pharmaceutical and medical device packaging. As regulatory expectations continue to emphasise data integrity and risk-based decision-making, deterministic CCIT methods have become the industry standard. These technologies provide quantitative, repeatable, and non-destructive results, offering clear advantages over traditional probabilistic methods.

Despite these advantages, deterministic CCIT systems can still generate false positives or false negatives if test methods are not scientifically developed and properly controlled. These inaccurate results directly affect batch disposition decisions, regulatory confidence, and ultimately patient safety. At PTI, reducing false results is a core focus of deterministic CCIT method development across the product lifecycle.

Impact of False Positives and False Negatives on Quality Decisions

False positives occur when a package that is integral is incorrectly identified as leaking. While often viewed as a conservative outcome, repeated false positives can create significant operational challenges. Manufacturers may experience unnecessary batch holds, increased scrap, delayed product release, and extended investigations that consume time and resources. Over time, this can reduce confidence in the CCIT method itself, particularly during audits where repeatability and objectivity are closely reviewed.

False negatives represent a more serious risk. In this case, a genuine integrity breach goes undetected, allowing compromised packages to pass testing. This can result in microbial ingress, loss of sterility, or product degradation due to oxygen or moisture exposure. For sterile injectables, biologics, and combination products, the consequences can include patient safety risks, regulatory findings, and product recalls. Regulators increasingly favor deterministic CCIT precisely because false negatives associated with probabilistic methods are difficult to measure and justify.

How PTI Deterministic Systems Reduce False Results Through Method Development

PTI approaches deterministic CCIT as a structured, science-based process rather than a simple compliance exercise. Method development begins with a clear definition of the critical leak size based on product risk, shelf-life requirements, and intended use. Test sensitivity is then aligned specifically to that risk, ensuring meaningful detection without unnecessary over-sensitivity.

A key element of PTI’s strategy is defect-based validation. By incorporating calibrated, known-size defects into method development, manufacturers can quantitatively verify detection limits and demonstrate repeatable performance. This approach provides strong scientific justification during regulatory inspections and significantly reduces uncertainty in test outcomes.

Technology selection and optimisation are also critical. PTI’s vacuum decay systems offer highly repeatable, non-destructive detection of gross to mid-sized leaks with minimal operator influence. Helium leak detection systems provide ultra-high sensitivity and precise leak rate measurement for applications requiring the highest level of assurance. Selecting the right technology for the application helps minimise ambiguous signals that often lead to false interpretations.

Deterministic data output further strengthens decision-making. Quantitative results allow for trend analysis, statistical control, and clear acceptance criteria, reducing reliance on subjective judgement. PTI also supports lifecycle integration, ensuring that CCIT methods developed during R&D remain relevant and robust as products move into clinical and commercial production.

Conclusion

False positives and false negatives in container closure integrity testing are not simply testing errors; they are quality risks with direct implications for patient safety, regulatory compliance, and operational efficiency. Deterministic CCI technologies provide a strong foundation, but their effectiveness depends on scientifically sound method development and validation.

Through risk-based sensitivity alignment, defect-centric validation, technology optimisation, and data-driven analysis, PTI helps manufacturers minimize false results and make confident, defensible quality decisions throughout the packaging lifecycle.

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ccit, package integrity testing, container closure integrity testing, cci technologies
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05
Jan 2026

Leak Size Determination in CCIT - Why Sensitivity Alone Is Not Enough

Leak-Size-Determination-in-CCIT-Why-Sensitivity-Alone-Is-Not-Enough

Container Closure Integrity Testing (CCIT) is essential for maintaining sterility, stability, and overall product quality in pharmaceutical and medical device packaging. With increasing adoption of deterministic CCIT methods, test sensitivity is often highlighted as a key performance indicator. However, sensitivity alone does not provide sufficient information to support meaningful quality decisions.

Leak size determination focuses on identifying defects that can realistically compromise product integrity during storage, distribution, and use. Without defining the relationship between detected leaks and product risk, CCIT results may indicate failure without clarifying whether the package no longer performs its intended protective function.

Difference Between Test Sensitivity and Critical Leak Size

Test sensitivity defines the smallest leak a test method can reliably detect under specified conditions. It is a characteristic of the measurement system and depends on factors such as resolution, test pressure or vacuum level, and system stability.

Critical leak size refers to the smallest defect that can result in loss of sterility, microbial ingress, or unacceptable levels of gas or moisture ingress over the product’s shelf life. This parameter is determined by the interaction between the product and the container closure system rather than the detection capability of the test method.

A CCIT method may detect leaks significantly smaller than the critical threshold. Without distinguishing between detectable leaks and functionally significant leaks, sensitivity alone can lead to results that are difficult to interpret and justify during batch disposition and regulatory review.

PTI Technologies Supporting Leak Size Determination

Deterministic CCI technologies from PTI Packaging Technologies are applied during method development and validation to support quantitative leak size analysis. Two technologies are commonly used for this purpose.

1. Vacuum Decay

Vacuum Decay is a non-destructive CCIT method that detects package leakage by measuring pressure changes within a sealed test chamber under vacuum conditions. When a leak is present, gas flows from the package into the chamber, producing a measurable pressure increase.

In leak size determination, Vacuum Decay is used to correlate pressure rise data with calibrated defect sizes. The method supports repeatable sensitivity studies and verification of detection capability at predefined leak thresholds. Because the test does not require tracer gas and preserves the package, it is suitable for stability studies and routine testing once acceptance criteria are established.

2. Helium Leak Detection

Helium Leak Detection using mass spectrometry is a quantitative CCIT method capable of measuring very small leak rates. Packages are filled or exposed to helium and tested under vacuum, where escaping helium is detected and quantified by a mass spectrometer.

For leak size determination, helium testing is used to measure leak rates associated with known defect sizes. This data enables direct correlation between physical leak size and potential ingress risk. Helium Leak Detection is commonly applied during development and validation to define critical leak size before translating requirements to other CCIT methods.

Conclusion

Sensitivity alone does not define the effectiveness of a CCIT strategy. Without determining critical leak size, test results may lack relevance to product quality and sterility assurance. Leak size determination establishes the connection between detectable defects and functional package performance.

Deterministic CCIT technologies such as Vacuum Decay and Helium Leak Detection enable quantitative analysis and risk-based acceptance criteria, supporting consistent and scientifically justified container closure integrity evaluation.

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ccit, package integrity testing, container closure integrity testing, cci technologies, helium leak detection
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Our technologies conform to ASTM and other regulatory standards.

Packaging Technologies & Inspection

PTI offers inspection systems for package leak testing, seal integrity and container closure integrity testing (CCIT). Our technologies exclude subjectivity from package testing, and use test methods that conform to ASTM standards. PTI's inspection technologies are deterministic test methods that produce quantitative test result data. We specialize in offering the entire solution including test method development and equipment validation.

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Packaging Technologies & Inspection

PTI offers inspection systems for package leak testing, seal integrity and container closure integrity testing (CCIT). Our technologies exclude subjectivity from package testing, and use test methods that conform to ASTM standards. PTI's inspection technologies are deterministic test methods that produce quantitative test result data. We specialize in offering the entire solution including test method development and equipment validation.

Sales Channel Partner Portal Login

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Our technologies conform to ASTM and other regulatory standards.

Get in Touch

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