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07
Aug 2025

Developing CCI Tests for Combination Products - Challenges and Strategies

Developing-CCI-Tests-for-Combination-Products-Challenges-and-Strategies

Combination products—therapeutic systems that combine two or more regulated components like drugs, devices, and biologics—are at the forefront of modern healthcare innovation. These products, such as prefilled syringes, auto-injectors, and inhalers, offer enhanced therapeutic benefits, improved patient compliance, and streamlined drug delivery. However, the complexity of these products presents unique regulatory and quality assurance challenges, especially in the context of Container Closure Integrity testing (CCIT). Ensuring container closure integrity is critical for maintaining product sterility, stability, and safety over its shelf life. This is particularly vital for combination products, where multiple components and interfaces increase the risk of contamination or leakage.

What Are Combination Products?

Combination products are therapeutic and diagnostic medical products that combine a drug, device, and/or biologic. According to the U.S. FDA, a combination product is defined as:

  • A product comprised of two or more regulated components that are physically, chemically, or otherwise combined and produced as a single entity (e.g., prefilled syringe, drug-eluting stent).
  • Two or more separate products packaged together in a single package or as a unit (e.g., co-packaged drug and device).
  • A drug, device, or biological product packaged separately that is intended for use only with another specified product (e.g., insulin pen with replaceable cartridges).
  • Because these products often require sterile delivery and involve intricate interfaces between components, ensuring robust sealing becomes an integral part of quality control.

Why Is CCI Testing of Combination Products Crucial?

Container Closure Integrity (CCI) testing verifies whether the packaging system can maintain a sterile barrier against potential contaminants such as microorganisms, oxygen, or moisture. In the case of combination products, CCI testing is particularly critical for several reasons:

  • Regulatory Requirements: Regulatory bodies like the FDA and EMA mandate integrity testing of sterile combination products. USP <1207> provides guidance on deterministic methods for assessing CCI.
  • Risk of Contamination: Multiple interfaces in combination products (e.g., between drug reservoir and delivery mechanism) pose higher risks of microbial ingress or leak paths.
  • Product Stability: Loss of container integrity can compromise drug potency, efficacy, and shelf life, especially in biologics and protein-based drugs
  • Patient Safety: Any breach in integrity can result in patient harm due to contamination, incorrect dosing, or compromised sterility.
  • Design Complexity: Irregular geometries, moving parts, and assembled components increase the difficulty of performing reliable integrity testing using traditional methods

Combination Products CCI Testing Using Helium Leak Detection Technology

Helium Leak Detection (HLD) has emerged as a gold standard among deterministic CCI testing methods, especially for complex and high-risk products like combination drug-device systems.

What Is Helium Leak Detection?

Helium leak testing is a valuable method for evaluating container closure integrity and detecting leaks in sealed systems. Helium, being a small and inert gas, is an ideal tracer gas for leak testing because it can easily escape from the smallest openings in a package or container. The process involves filling the package with helium and subjecting it to a vacuum, creating a pressure differential between the inside and outside of the package. If there are any leaks, helium will escape from the package, and its concentration outside the package can be measured using a helium leak detector. The concentration of helium detected is then quantified and expressed as a leak rate, providing valuable information about the package's integrity.

Helium leak testing finds extensive applications in various industries, especially in the pharmaceutical and parenteral products sector, where ensuring product containment is critical for maintaining product safety and efficacy. It can also be utilized in product design, quality analysis, failure analysis, and validation processes. Additionally, helium leak testing can help locate the specific points of leakage, which aids in identifying and resolving potential issues in the packaging design or manufacturing process.

Benefits of Helium Leak Detection Technology

Helium Leak Detection provides numerous advantages over traditional probabilistic methods like dye ingress or microbial ingress:

  • Deterministic and Quantitative: Unlike dye ingress testing, which relies on visual inspection and can be subjective, HLD offers precise and objective leak rate measurements.
  • High Sensitivity: HLD can detect extremely small leaks—orders of magnitude more sensitive than traditional test methods—making it ideal for high-risk sterile combination products.
  • Versatility Across Product Types” Whether it's prefilled syringes, pen injectors, or inhalers, HLD systems can be customized with fixtures and chambers to accommodate different product designs.
  • Regulatory Acceptance: HLD is endorsed by USP <1207> as a deterministic method and is widely accepted by regulatory authorities worldwide as a reliable means of CCI testing.
  • R&D and Root Cause Analysis: HLD enables accurate leak localization, making it valuable during R&D for design optimization and during investigations of package failures or complaints.

Combination products continue to drive innovation in drug delivery, but their complexity demands equally sophisticated quality assurance strategies. Ensuring container closure integrity is not just a regulatory necessity—it is a vital part of product performance and patient safety.

Helium Leak Detection technology stands out as an essential tool in the pharmaceutical and medical device sectors, offering unparalleled sensitivity, accuracy, and versatility. By integrating HLD early in the design and manufacturing process, companies can mitigate risks, reduce product failures, and deliver safer, more reliable combination products to the market.

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helium leak testing, helium leak detection, pharmaceutical packaging, helium leak detection solutions
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PTI offers inspection systems for package leak testing, seal integrity and container closure integrity testing (CCIT). Our technologies exclude subjectivity from package testing, and use test methods that conform to ASTM standards. PTI's inspection technologies are deterministic test methods that produce quantitative test result data. We specialize in offering the entire solution including test method development and equipment validation.

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Packaging Technologies & Inspection

PTI offers inspection systems for package leak testing, seal integrity and container closure integrity testing (CCIT). Our technologies exclude subjectivity from package testing, and use test methods that conform to ASTM standards. PTI's inspection technologies are deterministic test methods that produce quantitative test result data. We specialize in offering the entire solution including test method development and equipment validation.

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Our technologies conform to ASTM and other regulatory standards.

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